Factor VIII and von Willebrand factor are ligands for the carbohydrate-receptor Siglec-5
نویسندگان
چکیده
منابع مشابه
Factor VIII and von Willebrand factor are ligands for the carbohydrate-receptor Siglec-5.
BACKGROUND Factor VIII (FVIII) and von Willebrand factor (VWF) circulate in plasma in a tight non-covalent complex, being critical to hemostasis. Although structurally unrelated, both share the presence of sialylated glycan-structures, making them potential ligands for sialic-acid-binding-immunoglobulin-like-lectins (Siglecs). DESIGN AND METHODS We explored the potential interaction between F...
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The normal Factor VIII/von Willebrand factor protein has the ability to agglutinate or aggregate normal platelets in the presence of ristocetin (von Willebrand factor activity). Removal of greater than 95% of the sialic acid from this protein by neuraminidase did not affect the von Willebrand factor or procoagulant activity. However, oxidation of the penultimate galactose of the asialo Factor V...
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Factor VIII circulates in noncovalent complex with von Willebrand factor (vWf). The topography of this complex was evaluated by fluorescence energy transfer using factor VIII subunits modified with N-(1-pyrenyl)maleimide (NPM; fluorescence donor) and vWf-derived fragments modified with 7-diethylamino-3-[4'-maleimidylphenyl]-4-methyl coumarin (CPM; fluorescence acceptor). Results from a previous...
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ژورنال
عنوان ژورنال: Haematologica
سال: 2012
ISSN: 0390-6078,1592-8721
DOI: 10.3324/haematol.2012.063297